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Manali Shirke

Manali Shirke

Research Assistant-II

The Ohio State University

Research Assistant-II at The Ohio State University

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The Ohio State University

Research Assistant-II

Soybean tissue culture
Soybean Transformation- Agrobacterium and Biolistics

SDSU

Research Assistant

Protein extraction
Kheldal

SDSU

Graduate Resarch Assistant

Developing protocol for genetic transformation of wheat and rice
Training undergraduate students in the field of tissue culture and molecular biology since last 3 yrs.
Microscopy- Confocal, Fluorescent and Inverted
Histology- Embedding tissues in paraffin followed by sectioning using Microtomy,staining followed by microscopic observation
Molecular Biology: Cloning, PCR, Western Blot, Protein purification, Preparation of competent cells DH5alpha, DNA extraction followed by electrophoresis, SDS-PAGE,

Career medium 03
Career
Career medium 04

SDSU

Graduate Teaching Assistant

Conducting and teaching Biology labs for sophomores
Encouraging group discussion
Resolving queries via personal interaction and written correspondence
Grading exams

Career

Metropolis Health Services (I) Ltd

Senior Research Scientist

-Running assays on RT-PCR, Gene Sequencer, COBAS. Biological samples such as pulmonary as well as extra-pulmonary samples were tested for quantification of TB, HIV, HBV and HCV.
-Genotyping of positive HIV samples to study drug resistance.
-Quantification and detection of Factor V laden disease with the aid of analytical equipment.
-Bone marrow culturing along with karyotyping to study the chromosomal disorder.
-ELISA
-Extracting human DNA from wax embedded tissues
-Preparing and maintaining SOP
-Attended NABL and CAP Audits.

Career medium 06
Career
Career medium 03

Labnics Equipment

Technical Assistant – Team Lead

Researching suitable biotech equipment from different vendors which are in compliance with ISO
Scientific content writing
Managing Vendor, Content, Catalog & Web departments.

Career

SDSU

Master of Science (MS), Biological Sciences / Microbiology

PEXA, RUIA (UGC accrediated course)

Post Graduate Diploma, Clinico-Regulatory & Patents, Clinical Research

Mumbai University

MS, Biotechnology

Mumbai University

Bachelor of Science (BSc), Biotechnology